Journal: Brain, behavior, and immunity

Article Title: HIV-1 Tat protein enhances sensitization to methamphetamine by affecting dopaminergic function

doi: 10.1016/j.bbi.2017.05.004

Figure Lengend Snippet: Immunohistochemistry on paraffin embedded sections was utilized examine the protein distribution and levels of dopamine receptor D1 (A, B, C, D), dopamine receptor D2 (E, F, G, H), as well as of IBA-1 (I, J, K, L) in SAL TAT− (A, E, I), SAL TAT+ (B, F, J), METH TAT− (C, G, K), and METH TAT+ (D, H, L) mice. Representative positive cells in the 40× magnification images were labeled with a black arrow. (M) Normalized intensity density was calculated in ImageJ. Data are expressed as Mean ± SEM (n=5). * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: Sections were blocked with 5g/l Casein (Sigma Aldrich) in PBS, containing 0.5g/l Thimerosal (Sigma Aldrich) and incubated with Iba-1 antibody (Wako Lab Chemicals, Richmond, VA), the anti-mouse DRD1 antibody (NLS43, Novus Biologicals, Littleton, CO), or anti mouse DRD2 (orb154598, Biorbyt, San Francisco, CA), each one diluted in Casein buffer.

Techniques: Immunohistochemistry, Labeling

Journal: Reproductive biomedicine online

Article Title: Social psychogenic stress promotes the development of endometriosis in mouse.

doi: 10.1016/j.rbmo.2016.11.012

Figure Lengend Snippet: Figure 4 – Immmunoreactivity staining of different markers in ectopic lesions in different groups. (A) Representative immunostaining of ADRB2 in endometrium in CONTROL and SHAM mice and in ectopic endometrium in STRESSED and UNSTRESSED mice. ADRB2 and DRD2 immunoreactivity was both seen primarily in glandular epithelial cells and was localized in the cytoplasm. Scale bar = 125 μm. (B) Representative immunostaining of DRD2, VEGF, CD31, CD41, F4/80, PCNA and α-SMA in the ectopic lesions in UNSTRESSED and STRESSED groups. VEGF immunoreactivity was seen primarily in glandular epithelial cells and was localized in the cytoplasm. CD31 immunostainings were seen mostly in vascular endothelial cells. CD41 shows the extent of platelet aggregation and F4/80 immunoreactivity represents the extent of macrophage infiltration. PCNA immunoreactivity was seen both in glandular epithelial cells and stromal cells were localized in the cell nucleus, but the change of immunoreactivity in glandular epithelial cells was more obvious. α-SMA staining were seen mostly in the stromal component of the ectopic lesions. Scale bar = 125 μm. Mice in stressed (STRS) and unstressed (UNSTRS) groups had undergone endometriosis-inducing surgery, while mice the SHAM group underwent non-endometriosis-inducing surgery. Mice in unstressed (UNSTRS), SHAM and control (CTL) groups were not exposed to stress.

Article Snippet: For negative controls, the immunoglobulin G (IgG) from the rabbit serum (Sigma, Darmstadt, Germany) was used instead of primary antibodies against ADRB2, DRD2, VEGF, CD31, CD41, α-SMA and PCNA except for F4/80, for which the IgG was from the rat serum (Boster, Wuhan, China) was used, all used at the same concentration with its corresponding primary antibody.

Techniques: Staining, Immunostaining, Control

Journal: Biology of Sex Differences

Article Title: Sex difference in dopamine D1-D2 receptor complex expression and signaling affects depression- and anxiety-like behaviors

doi: 10.1186/s13293-020-00285-9

Figure Lengend Snippet: Comparison of the expression of dopamine D1-D2 receptor heteromer between male and female rats by co-immunoprecipitation (Co-IP) and proximity ligation assay (PLA). a Co-IP of D1R with anti-D2R antibody from NAc and CPu of male (M) and female (F) rats, followed by western blot to visualize D1R. b Western blot analysis of D1R in NAc and CPu in both sexes. c Western blot analysis of D2R in NAc and CPu of both sexes. d Quantification of Co-IP and WB results from a – c . N = 3–4 rats/group for Co-IP and N = 6 rats/group for WB. * p < 0 . 05 , ** p < 0.01 using t test. e Confocal images and insets (left) and quantification (right) of D1-D2 PLA signal in NAc and CPu of male and female rat. N = 6 rats/group, ANOVA, followed by Bonferroni’s post-test comparisons. ** p < 0 . 001 , *** p < 0 . 0001 . Data are expressed as means ± SEM. f Confocal images (left) and quantification (right) of D1-D2 PLA signal in the caudate and putamen nuclei of male and female African Green monkey. N = 3 animals/group. *** p < 0 . 0001 using t test. Data are expressed as means ± SEM

Article Snippet: Protein homogenates (250–300 μg) from rat NAc or CPu were incubated with an anti-D2R antibody (Alomone Laboratories) at 4 °C overnight under gentle rotation.

Techniques: Expressing, Immunoprecipitation, Co-Immunoprecipitation Assay, Proximity Ligation Assay, Western Blot

Journal: Advanced materials (Deerfield Beach, Fla.)

Article Title: Intranasal Delivery of Near-infrared and Magnetic Dual-response Nanospheres to Rapidly Produce Antidepressant-like and Cognitive Enhancement Effects.

doi: 10.1002/adma.202405547

Figure Lengend Snippet: Figure 7. Investigation of the mechanism of therapeutic effect and biosafety assessment in vivo. a) Fluorescence staining of different brain regions. Blue: 4′,6-diamidino-2-phenylindole (DAPI), Red: TH or DRD2 or DRD1. The circle statistical charts show the fluorescence intensity ratio. b) Fluorescence intensity ratio of DRD2 or DRD1 in the PFC. c) Representative immunoblots of proteins in dopamine-related signaling pathways. d–l) Quantitative analyses of proteins in (c). Data are means ± s.d. (n ≥3), *p < 0.05, **p < 0.01.

Article Snippet: TH, DRD1, and DRD2 polyclonal antibodies were purchased from Proteintech Group, Inc. All other reagents were purchased from commercial resources and used as received unless otherwise noted.

Techniques: In Vivo, Fluorescence, Staining, Western Blot, Protein-Protein interactions

Journal: International journal of molecular sciences

Article Title: Total Alkaloid Extract of Nelumbinis Plumula Promoted Sleep in PCPA-Induced Insomnia Rats by Affecting Neurotransmitters and Their Receptor Activities.

doi: 10.3390/ijms26083684

Figure Lengend Snippet: Figure 9. The effects of NP, NPA, and NPP extracts on GABRA1 and AChE proteins in hippocampus of PCPA-induced insomnia rats (n = 3) (magnification × 20, scale bar = 50 µm). (A) The effects in the CA1 area of hippocampus. (B) The effects in the CA3 area of hippocampus. (C) Quantification of the protein expression of GABRA1 in the CA1 area (x ± S) (n = 6). (D) Quantification of the protein expression of AChE in the CA1 area (x ± S) (n = 6). (E) Quantification of the protein expression of GABRA1 in the CA3 area (x ± S) (n = 6). (F) Quantification of the protein expression of AChE in the CA3 area (x ± S) (n = 6). Compared with the control group, #: p < 0.05; Compared with the model group, *: p < 0.05, **: p < 0.01, ***: p < 0.001.

Article Snippet: The GABRA1 (14471-1-AP) and DRD2 (55084- 1-AP) primary antibodies were purchased from Proteintech Group, Inc. (Wuhan, Hubei, China).

Techniques: Expressing, Control